[HTML][HTML] APOL1 renal risk variants exacerbate podocyte injury by increasing inflammatory stress
H Wakashin, J Heymann, H Roshanravan… - BMC nephrology, 2020 - Springer
BMC nephrology, 2020•Springer
Abstract Background Apolipoprotein L1, APOL1, is a trypanosome lytic factor present in
human and certain other primates. APOL1 gene variants, present in individuals of recent sub-
Saharan African descent, increase risk for glomerular disease and associate with the
disease progression, but the molecular mechanisms have not been defined. Objectives We
focus on the mechanism how APOL1 variant proteins enhance podocyte injury in the
stressed kidney. Methods First, we investigated the expression of APOL1 protein isoform …
human and certain other primates. APOL1 gene variants, present in individuals of recent sub-
Saharan African descent, increase risk for glomerular disease and associate with the
disease progression, but the molecular mechanisms have not been defined. Objectives We
focus on the mechanism how APOL1 variant proteins enhance podocyte injury in the
stressed kidney. Methods First, we investigated the expression of APOL1 protein isoform …
Background
Apolipoprotein L1, APOL1, is a trypanosome lytic factor present in human and certain other primates. APOL1 gene variants, present in individuals of recent sub-Saharan African descent, increase risk for glomerular disease and associate with the disease progression, but the molecular mechanisms have not been defined.
Objectives
We focus on the mechanism how APOL1 variant proteins enhance podocyte injury in the stressed kidney.
Methods
First, we investigated the expression of APOL1 protein isoform and the localization of APOL1 protein in the kidney. Next, we examined the role of APOL1 in the podocyte stress and the inflammatory signaling in the kidney after hemi-nephrectomy.
Results
We identified a novel RNA variant that lacks a secretory pathway signal sequence and we found that the predicted APOL1-B3 protein isoform was expressed in human podocytes in vivo and by BAC-APOL1 transgenic mice. APOL1-B3-G2 transgenic mice, carrying a renal risk variant, manifested podocyte injury and increased pro-IL-1β mRNA in isolated glomeruli and increased IL-1β production in the remnant kidney after uninephrectomy. APOL1-B3 interacted with NLRP12, a key regulator of Toll-like receptor signaling.
Conclusions
These results suggest a possible mechanism for podocyte injury by which one of the APOL1 protein isoforms, APOL1-B3 and its renal risk variants, enhances inflammatory signaling.
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