Transcriptional activation of the heme oxygenase gene by heme and cadmium in mouse hepatoma cells
J Alam, S Shibahara, A Smith - Journal of Biological Chemistry, 1989 - ASBMB
Treatment of mouse hepatoma (Hepa) cells with heme or cadmium chloride in serum-free
medium causes a rapid increase in the steady-state level of heme oxygenase (HO)
messenger RNA. This increase is both dose-and time-dependent. Maximum accumulation of
HO mRNA is observed 3 h after addition of either agent. Treatment of Hepa cells with heme
or CdCl 2 also stimulates the transcription of the HO gene, as judged by in vitro nuclear
transcription run-on assays. The maximum rate of HO gene transcription occurs 2 h after …
medium causes a rapid increase in the steady-state level of heme oxygenase (HO)
messenger RNA. This increase is both dose-and time-dependent. Maximum accumulation of
HO mRNA is observed 3 h after addition of either agent. Treatment of Hepa cells with heme
or CdCl 2 also stimulates the transcription of the HO gene, as judged by in vitro nuclear
transcription run-on assays. The maximum rate of HO gene transcription occurs 2 h after …
