[PDF][PDF] Differential expression of Ly6C and T-bet distinguish effector and memory Th1 CD4+ cell properties during viral infection

HD Marshall, A Chandele, YW Jung, H Meng… - Immunity, 2011 - cell.com
HD Marshall, A Chandele, YW Jung, H Meng, AC Poholek, IA Parish, R Rutishauser, W Cui
Immunity, 2011cell.com
CD4+ T cells differentiate into multiple effector types, but it is unclear how they form memory
T cells during infection in vivo. Profiling virus-specific CD4+ T cells revealed that effector
cells with T helper 1 (Th1) or T follicular helper (Tfh) cell characteristics differentiated into
memory cells, although expression of Tfh cell markers declined over time. In contrast to virus-
specific effector CD8+ T cells, increased IL-7R expression was not a reliable marker of
CD4+ memory precursor cells. However, decreased Ly6C and T-bet (Tbx21) expression …
Summary
CD4+ T cells differentiate into multiple effector types, but it is unclear how they form memory T cells during infection in vivo. Profiling virus-specific CD4+ T cells revealed that effector cells with T helper 1 (Th1) or T follicular helper (Tfh) cell characteristics differentiated into memory cells, although expression of Tfh cell markers declined over time. In contrast to virus-specific effector CD8+ T cells, increased IL-7R expression was not a reliable marker of CD4+ memory precursor cells. However, decreased Ly6C and T-bet (Tbx21) expression distinguished a subset of Th1 cells that displayed greater longevity and proliferative responses to secondary infection. Moreover, the gene expression profile of Ly6CloT-betint Th1 effector cells was virtually identical to mature memory CD4+ T cells, indicating early maturation of memory CD4+ T cell features in this subset during acute viral infection. This study provides a framework for memory CD4+ T cell development after acute viral infection.
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