Urea-induced ROS cause endothelial dysfunction in chronic renal failure

M D'Apolito, X Du, D Pisanelli, M Pettoello-Mantovani… - Atherosclerosis, 2015 - Elsevier
Objective The pathogenic events responsible for accelerated atherosclerosis in patients with
chronic renal failure (CRF) are poorly understood. Here we investigate the hypothesis that
concentrations of urea associated with CRF and increased ROS production in adipocytes
might also increase ROS production directly in arterial endothelial cells, causing the same
pathophysiologic changes seen with hyperglycemia. Methods Primary cultures of human
aortic endothelial cells (HAEC) were exposed to 20 mM urea for 48 h. C57BL/6J wild-type …