[HTML][HTML] The Rad9-Hus1-Rad1 checkpoint clamp regulates interaction of TopBP1 with ATR
TopBP1 serves as an activator of the ATR-ATRIP complex in response to the presence of
incompletely replicated or damaged DNA. This process involves binding of ATR to the ATR-
activating domain of TopBP1, which is located between BRCT domains VI and VII. TopBP1
displays increased binding to ATR-ATRIP in Xenopus egg extracts containing checkpoint-
inducing DNA templates. We show that an N-terminal region of TopBP1 containing BRCT
repeats I-II is essential for this checkpoint-stimulated binding of TopBP1 to ATR-ATRIP. The …
incompletely replicated or damaged DNA. This process involves binding of ATR to the ATR-
activating domain of TopBP1, which is located between BRCT domains VI and VII. TopBP1
displays increased binding to ATR-ATRIP in Xenopus egg extracts containing checkpoint-
inducing DNA templates. We show that an N-terminal region of TopBP1 containing BRCT
repeats I-II is essential for this checkpoint-stimulated binding of TopBP1 to ATR-ATRIP. The …