Inducible podocyte injury and proteinuria in transgenic zebrafish

W Zhou, F Hildebrandt - Journal of the American Society of …, 2012 - journals.lww.com
Damage or loss of podocytes causes glomerulosclerosis in murine models, and mutations in
podocyte-specific genes cause nephrotic syndrome in humans. Zebrafish provide a valuable
model for kidney research, but disruption of pronephroi leads to death within a few days,
thereby preventing the study of CKD. In this study, we generated an inducible model of
podocyte injury in zebrafish (pod:: NTR-mCherry) by expressing a bacterial nitroreductase,
which converts metronidazole to a cytotoxin, specifically in podocytes under the control of …