[HTML][HTML] Stress-activated protein kinase-2/p38 and a rapamycin-sensitive pathway are required for C2C12 myogenesis
The differentiation of C2C12 myoblasts to myotubes was found to be accompanied by a
strong activation of p70 S6 kinase and the mitogen-activated protein kinase (MAPK) family
member SAPK2/p38, without significant activation of p42 MAPK and only slight activation of
SAPK1/JNK and protein kinase Bα. Consistent with these findings, SB 203580 (a specific
inhibitor of SAPK2/p38) or rapamycin (which blocks the activation of p70 S6 kinase)
prevented the formation of multinucleated myotubes, as well as the expression of muscle …
strong activation of p70 S6 kinase and the mitogen-activated protein kinase (MAPK) family
member SAPK2/p38, without significant activation of p42 MAPK and only slight activation of
SAPK1/JNK and protein kinase Bα. Consistent with these findings, SB 203580 (a specific
inhibitor of SAPK2/p38) or rapamycin (which blocks the activation of p70 S6 kinase)
prevented the formation of multinucleated myotubes, as well as the expression of muscle …