Employing in vitro pulse-chase techniques, we investigated the de novo synthesis and the
kinetics of intracellular transport and extracellular matrix incorporation of proteoglycans (PG)
by normal human renal epithelial cell and by epithelial cells isolated from cysts of autosomal
dominant kidneys (ADPKD). Cell monolayers were pulsed either with (3H) leucine for 15 min
and chased for seven intervals between 15 and 270 min or with (35S) sulfate for 150 min
and chased for a single interval of 120 min. Total proteins and PG were isolated from cell …