[HTML][HTML] Prolonged kappa opioid receptor phosphorylation mediated by G-protein receptor kinase underlies sustained analgesic tolerance
JP McLaughlin, LC Myers, PE Zarek, MG Caron… - Journal of Biological …, 2004 - ASBMB
Kappa opioid receptor (KOR) desensitization was previously shown to follow agonist-
dependent phosphorylation of serine 369 by G-protein receptor kinase (GRK) and β-arrestin
binding in transfected cells. To study the in vivo effects induced by phosphorylation of KOR
(S369), C57Bl/6 mice were administered single or repeated doses of the KOR agonist, U50,
488, and isolated brain glycoprotein was probed with an antibody, KOR-P, that specifically
recognized phosphoserine 369 KOR. Western blot analysis using KOR-P antibody showed …
dependent phosphorylation of serine 369 by G-protein receptor kinase (GRK) and β-arrestin
binding in transfected cells. To study the in vivo effects induced by phosphorylation of KOR
(S369), C57Bl/6 mice were administered single or repeated doses of the KOR agonist, U50,
488, and isolated brain glycoprotein was probed with an antibody, KOR-P, that specifically
recognized phosphoserine 369 KOR. Western blot analysis using KOR-P antibody showed …
