• NO and oxyradical metabolism in new cell lines of rat brain capillary endothelial cells forming the blood–brain barrier
IE Blasig, H Giese, ML Schroeter, A Sporbert… - Microvascular …, 2001 - Elsevier
To investigate the relevance of• NO and oxyradicals in the blood–brain barrier (BBB),
differentiated and well-proliferating brain capillary endothelial cells (BCEC) are required.
Therefore, rat BCEC (rBCEC) were transfected with immortalizing genes. The resulting lines
exhibited endothelial characteristics (factor VIII, angiotensin-converting enzyme, high
prostacyclin/thromboxane release rates) and BBB markers (γ-glutamyl transpeptidase,
alkaline phosphatase). The control line rBCEC2 (mock transfected) revealed fibroblastoid …
differentiated and well-proliferating brain capillary endothelial cells (BCEC) are required.
Therefore, rat BCEC (rBCEC) were transfected with immortalizing genes. The resulting lines
exhibited endothelial characteristics (factor VIII, angiotensin-converting enzyme, high
prostacyclin/thromboxane release rates) and BBB markers (γ-glutamyl transpeptidase,
alkaline phosphatase). The control line rBCEC2 (mock transfected) revealed fibroblastoid …
