[HTML][HTML] Insulin and sterol-regulatory element-binding protein-1c (SREBP-1c) regulation of gene expression in 3T3-L1 adipocytes: identification of CCAAT/enhancer …
S Le Lay, I Lefrere, C Trautwein, I Dugail… - Journal of Biological …, 2002 - ASBMB
We evaluated the hypothesis of sterol-regulatory element-binding protein (SREBP)-1c being
a general mediator of the transcriptional effects of insulin, with a focus on adipocytes, in
which insulin profoundly influences specific gene expression. Using real time quantitative
reverse transcriptase-PCR to monitor changes in the expression of about 50 genes that
cover a wide range of adipocyte functions, we have compared the impact of insulin treatment
with that of adenoviral overexpression of either dominant positive or dominant negative …
a general mediator of the transcriptional effects of insulin, with a focus on adipocytes, in
which insulin profoundly influences specific gene expression. Using real time quantitative
reverse transcriptase-PCR to monitor changes in the expression of about 50 genes that
cover a wide range of adipocyte functions, we have compared the impact of insulin treatment
with that of adenoviral overexpression of either dominant positive or dominant negative …
