[CITATION][C] Isolation of an organ specific protein antigen from cell-surface membrane rat liver
DM Neville Jr - Biochimica et Biophysica Acta (BBA)-Protein Structure, 1968 - Elsevier
1. A single membrane protein accounting for roughly 10% of the total membrane protein has
been isolated from alkali extracts of rat liver cell membranes by preparative disc
electrophoresis at pH 2.7 in urea. 2. 2. A Stokes radius of 58 Å and a molecular weight of 70
000 has been estimated for the protein by gel filtration in 8 M urea. 3. 3. Divalent cations are
involved in the binding of the protein to the membrane matrix. Dialysis of membranes
against 1 mM EDTA solubilizes the protein at neutral pH. 4. 4. Antisera prepared against the …
been isolated from alkali extracts of rat liver cell membranes by preparative disc
electrophoresis at pH 2.7 in urea. 2. 2. A Stokes radius of 58 Å and a molecular weight of 70
000 has been estimated for the protein by gel filtration in 8 M urea. 3. 3. Divalent cations are
involved in the binding of the protein to the membrane matrix. Dialysis of membranes
against 1 mM EDTA solubilizes the protein at neutral pH. 4. 4. Antisera prepared against the …
