Expression of two novel eph-related receptor protein tyrosine kinases in mammary gland development and carcinogenesis.
AC Andres, HH Reid, G Zürcher, RJ Blaschke… - Oncogene, 1994 - europepmc.org
AC Andres, HH Reid, G Zürcher, RJ Blaschke, D Albrecht, A Ziemiecki
Oncogene, 1994•europepmc.orgTo investigate the involvement of protein tyrosine kinases (PTKs) in the growth control of
mammary epithelial cells, we have used PCR based cloning to identify PTKs expressed in a
mouse mammary epithelial cell line. This approach led to the isolation of two receptor PTKs
of the eph-related subfamily; myk-1, a novel member expressed predominantly in lung, heart
and mammary gland and myk-2, a close relative of the human eck gene. Northern blot
analysis of RNA from mouse mammary glands at different stages of development revealed …
mammary epithelial cells, we have used PCR based cloning to identify PTKs expressed in a
mouse mammary epithelial cell line. This approach led to the isolation of two receptor PTKs
of the eph-related subfamily; myk-1, a novel member expressed predominantly in lung, heart
and mammary gland and myk-2, a close relative of the human eck gene. Northern blot
analysis of RNA from mouse mammary glands at different stages of development revealed …
To investigate the involvement of protein tyrosine kinases (PTKs) in the growth control of mammary epithelial cells, we have used PCR based cloning to identify PTKs expressed in a mouse mammary epithelial cell line. This approach led to the isolation of two receptor PTKs of the eph-related subfamily; myk-1, a novel member expressed predominantly in lung, heart and mammary gland and myk-2, a close relative of the human eck gene. Northern blot analysis of RNA from mouse mammary glands at different stages of development revealed that myk-1 and myk-2 expression is induced at puberty and differentially regulated during the estrus cycle. myk-1 and myk-2 expression was down-regulated during the pregnancy induced differentiation of the mammary gland. Over-expression of myk-1 and myk-2 was found in the undifferentiated and invasive mammary tumors of transgenic mice expressing the Ha-ras oncogene. In contrast, no elevated expression of either gene could be detected in the well differentiated and non-metastatic mammary tumors of c-myc expressing transgenic mice. These results indicate that myk-1 and myk-2 expression is induced during the proliferation of the mammary gland and down-regulated by its differentiation.
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