[HTML][HTML] The large conductance, voltage-dependent, and calcium-sensitive K+ channel, Hslo, is a target of cGMP-dependent protein kinase phosphorylation in vivo
A Alioua, Y Tanaka, M Wallner, F Hofmann… - Journal of Biological …, 1998 - ASBMB
Native large conductance, voltage-dependent, and Ca 2+-sensitive K+ channels are
activated by cGMP-dependent protein kinase. Two possible mechanisms of kinase action
have been proposed: 1) direct phosphorylation of the channel and 2) indirect via PKG-
dependent activation of a phosphatase. To scrutinize the first possibility, at the molecular
level, we used the human pore-forming α-subunit of the Ca 2+-sensitive K+ channel, Hslo,
and the α-isoform of cGMP-dependent protein kinase I. In cell-attached patches of oocytes …
activated by cGMP-dependent protein kinase. Two possible mechanisms of kinase action
have been proposed: 1) direct phosphorylation of the channel and 2) indirect via PKG-
dependent activation of a phosphatase. To scrutinize the first possibility, at the molecular
level, we used the human pore-forming α-subunit of the Ca 2+-sensitive K+ channel, Hslo,
and the α-isoform of cGMP-dependent protein kinase I. In cell-attached patches of oocytes …