Increased collagen accumulation in dermal fibroblast cultures from patients with progressive systemic sclerosis (scleroderma)
RB Buckingham, RK Prince… - The Journal of …, 1978 - translationalres.com
Fibroblast monolayers were established by explant culture of dermis from patients with PSS
and from normal individuals. Prior to explanting, each of the biopsies was split into three
fragments, so that monolayers were derived from a full-thickness portion of dermis (including
epidermis and subcutis), an upper portion of dermis (including epidermis), and a lower
portion of dermis (including subcutis). Collagen accumulation in the culture medium was
measured as 3 H-proline incorporation into CSP and as labeled hydroxyproline. Cultures …
and from normal individuals. Prior to explanting, each of the biopsies was split into three
fragments, so that monolayers were derived from a full-thickness portion of dermis (including
epidermis and subcutis), an upper portion of dermis (including epidermis), and a lower
portion of dermis (including subcutis). Collagen accumulation in the culture medium was
measured as 3 H-proline incorporation into CSP and as labeled hydroxyproline. Cultures …
