[HTML][HTML] cDNA cloning, expression, mutagenesis, intracellular localization, and gene chromosomal assignment of mouse 5-lipoxygenase
XS Chen, TA Naumann, U Kurre, NA Jenkins… - Journal of Biological …, 1995 - ASBMB
5-Lipoxygenase of mouse macrophages and bone marrow-derived mast cells (BMMC) was
investigated. Indirect immunocytofluorescence combined with confocal microscopy provided
evidence for distinct intracellular expression patterns and trafficking of 5-lipoxygenase upon
cellular activation. In resting BMMC, 5-lipoxygenase was found within the nucleus co-
localizing with the nuclear stain Yo-Pro-1. When BMMC were IgE/antigen-activated the 5-
lipoxygenase immunofluorescence pattern was changed from nuclear to perinuclear. The …
investigated. Indirect immunocytofluorescence combined with confocal microscopy provided
evidence for distinct intracellular expression patterns and trafficking of 5-lipoxygenase upon
cellular activation. In resting BMMC, 5-lipoxygenase was found within the nucleus co-
localizing with the nuclear stain Yo-Pro-1. When BMMC were IgE/antigen-activated the 5-
lipoxygenase immunofluorescence pattern was changed from nuclear to perinuclear. The …
