[HTML][HTML] NADH oxidase activity of mitochondrial apoptosis-inducing factor
MD Miramar, P Costantini, L Ravagnan… - Journal of Biological …, 2001 - ASBMB
Apoptosis-inducing factor (AIF) is a mitochondrial flavoprotein, which translocates to the
nucleus during apoptosis and causes chromatin condensation and large scale DNA
fragmentation. Here we report the biochemical characterization of AIF's redox activity.
Natural AIF purified from mitochondria and recombinant AIF purified from bacteria (AIFΔ1–
120) exhibit NADH oxidase activity, whereas superoxide anion (O 2−) is formed. AIFΔ1–120
is a monomer of 57 kDa containing 1 mol of noncovalently bound FAD/mol of protein …
nucleus during apoptosis and causes chromatin condensation and large scale DNA
fragmentation. Here we report the biochemical characterization of AIF's redox activity.
Natural AIF purified from mitochondria and recombinant AIF purified from bacteria (AIFΔ1–
120) exhibit NADH oxidase activity, whereas superoxide anion (O 2−) is formed. AIFΔ1–120
is a monomer of 57 kDa containing 1 mol of noncovalently bound FAD/mol of protein …
