[HTML][HTML] Nerve growth factor-induced neuronal differentiation requires generation of Rac1-regulated reactive oxygen species
K Suzukawa, K Miura, J Mitsushita, J Resau… - Journal of Biological …, 2000 - ASBMB
Nerve growth factor (NGF) stimulation of pheochromocytoma PC12 cells transiently
increased the intracellular concentration of reactive oxygen species (ROS). This increase
was blocked by the chemical antioxidant N-acetylcysteine and a flavoprotein inhibitor,
diphenylene iodonium. NGF responses of PC12 cells, including neurite outgrowth, tyrosine
phosphorylation, and AP-1 activation, was inhibited when ROS production was prevented
byN-acetylcysteine and diphenylene iodonium. The expression of dominant negative …
increased the intracellular concentration of reactive oxygen species (ROS). This increase
was blocked by the chemical antioxidant N-acetylcysteine and a flavoprotein inhibitor,
diphenylene iodonium. NGF responses of PC12 cells, including neurite outgrowth, tyrosine
phosphorylation, and AP-1 activation, was inhibited when ROS production was prevented
byN-acetylcysteine and diphenylene iodonium. The expression of dominant negative …
