Structure of the lipid-linked oligosaccharides that accumulate in class E Thy-1-negative mutant lymphomas
A Chapman, IS Trowbridge, R Hyman, S Kornfeld - Cell, 1979 - cell.com
A Chapman, IS Trowbridge, R Hyman, S Kornfeld
Cell, 1979•cell.comStudies reported in the preceding paper (Trowbridge and Hyman, 1979) have demonstrated
that Thy-l-mutant lymphoma cells of the class E complementation group lack the normal high
molecular weight lipid-linked oligosaccharide, but instead accumulate two smaller species
termed I and II. This paper reports studies which elucidate the structures of lipid-linked
oligosaccharides I and II. By subjecting oligosaccharides radiolabeled with 3H-mannose, 3H-
glucose or 3H-glucosamine to methylation, acetolysis, periodate oxidation and …
that Thy-l-mutant lymphoma cells of the class E complementation group lack the normal high
molecular weight lipid-linked oligosaccharide, but instead accumulate two smaller species
termed I and II. This paper reports studies which elucidate the structures of lipid-linked
oligosaccharides I and II. By subjecting oligosaccharides radiolabeled with 3H-mannose, 3H-
glucose or 3H-glucosamine to methylation, acetolysis, periodate oxidation and …
Summary
Studies reported in the preceding paper (Trowbridge and Hyman, 1979) have demonstrated that Thy-l-mutant lymphoma cells of the class E complementation group lack the normal high molecular weight lipid-linked oligosaccharide, but instead accumulate two smaller species termed I and II. This paper reports studies which elucidate the structures of lipid-linked oligosaccharides I and II. By subjecting oligosaccharides radiolabeled with 3H-mannose, 3H-glucose or 3H-glucosamine to methylation, acetolysis, periodate oxidation and exoglycosidase digestion, the structures were shown to be:
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